purified human platelet tsp1  (Haematologic Technologies)

Journal: Liver International

Article Title: The CD47-binding peptide of thrombospondin-1 induces defenestration of liver sinusoidal endothelial cells

doi: 10.1111/liv.12231

Figure Lengend Snippet: Thrombospondin 1 (TSP1) induced LSEC defenestration. (A) SEM images of LSEC after treatment with different doses (0 ng/ml, 10 ng/ml and 100 ng/ml) of TSP1 for 12 h. Scale bars represent 1 μm. White asterisks (*) indicate fenestrae or clusters of fenestrae (sieve plates). (B) Porosity/μm 2 (black bars, represent fenestrae area) and Fenestrae number/μm 2 (white bars, represent the absolute number of fenestrae) observed in LSEC after treatment with different doses of TSP1 (* represents P < 0.05 with Student's t -test).

Article Snippet: To show the long-term, general effect of TSP1 on LSECs, cells in were treated for 12 h with different doses (0 ng/ml, 10 ng/ml and 100 ng/ml) of purified human platelet TSP1 (Millipore, Billerica, MA, USA) in basal M131 medium and were fixed for SEM preparation.

Techniques:

Journal: Liver International

Article Title: The CD47-binding peptide of thrombospondin-1 induces defenestration of liver sinusoidal endothelial cells

doi: 10.1111/liv.12231

Figure Lengend Snippet: Thrombospondin 1 (TSP1)-induced defenestration was independent of TGF-β1 signalling. (A) SEM images of liver sinusoidal endothelial cells (LSEC)s pretreated with SB-431542 for 1 h before addition of TGF-β1 or TSP1 (a) Control; (b) SB-431542; (c) TSP1; (d) SB-431542+TSP1; (e) TGF-β1; (f) SB-431542+TGF-β1. All scale bars are 1 μm. (B) Porosity/μm 2 and (C) Fenestrae number/μm 2 after treatment with SB-431542, TSP1 and TGF-β1 (* represents P < 0.05 for one-way anova applied to the control, TSP1, and SB-431542+TSP1 treatments).

Article Snippet: To show the long-term, general effect of TSP1 on LSECs, cells in were treated for 12 h with different doses (0 ng/ml, 10 ng/ml and 100 ng/ml) of purified human platelet TSP1 (Millipore, Billerica, MA, USA) in basal M131 medium and were fixed for SEM preparation.

Techniques: Control

Journal: Liver International

Article Title: The CD47-binding peptide of thrombospondin-1 induces defenestration of liver sinusoidal endothelial cells

doi: 10.1111/liv.12231

Figure Lengend Snippet: Defenestration was induced by a peptide from the Thrombospondin 1 (TSP)1 C-terminal domain. (A) Domains of TSP1 and the origins of the recombinant peptides from the C-terminal binding domain (CBD). Amino acid residues differing from the native TSP1 residues are underlined. (B) Porosity/μm 2 and (C) Fenestrae number/μm 2 of liver sinusoidal endothelial cells (LSEC) after treatment with CBD peptides. (D) Distribution of LSEC fenestrae diameter after peptide treatment. (* represents P < 0.05 for one-way anova applied to Control, p4N1 and p4G1 treatments).

Article Snippet: To show the long-term, general effect of TSP1 on LSECs, cells in were treated for 12 h with different doses (0 ng/ml, 10 ng/ml and 100 ng/ml) of purified human platelet TSP1 (Millipore, Billerica, MA, USA) in basal M131 medium and were fixed for SEM preparation.

Techniques: Recombinant, Binding Assay, Control